This basin-wide research, prioritization, and design project improved tools and data for Pacific lamprey recovery across the Sacramento River system. Work included extending the state’s Pacific lamprey distribution GIS layer to include third-order streams in the Sacramento Basin, refining a standardized field barrier assessment form, using FishPass software to prioritize barriers, and developing passage modification designs for three high-priority sites. The Sacramento Basin has been severely impacted by impassable dams limiting lamprey to roughly half their historical range, and this project advances the integration of lamprey needs into California’s broader fish passage planning framework.